The Ossila Spectrometer is versatile and designed for performing optical spectroscopy across the entire visible spectrum.
This is the equipment set up you will need to do a transmission and absorption measurement.
Turn on the light by plugging the USB cable into the back.
Open the software and start a continuous measurement. Adjust the integration time so that a clear signal is visible (approximately 50,000 counts).
Transmission and absorption measurements are susceptible to noise, so increase the number of averages to reduce the noise.
Unplug the light source and take a background measurement.
Turn on the light, insert a blank sample if required, and take a reference spectrum.
Place the sample in the holder.
The background and reference spectra can now be hidden.
Change measurement type to ‘Transmission/Reflection’ and take a single measurement.
Right click on the plot and zoom in on the axis to view the spectrum.
Absorption measurements are made in the same way but selecting ‘Absorption’ as the measurement type.
In addition, measurements can be performed on liquids using the cuvette holder.